Master/Diploma Thesis in Molecular Cell Biology

BRAIN – Biotechnology Research and Information Network – was founded in 1993 in Zwingenberg (Rhine-Neckar region) and is now a leading biotech company in the industrial ‚white‘ biotechnology sector in Europe. BRAIN has developed a number of new bioactive products, microbial producer strains as well as a variety of enzymes and biocatalysts for its customers in the chemical and pharmaceutical as well as in the food and cosmetic industries. BRAIN regularly incorporates life science students into project work within the company. This work always accompanies the completion of a diploma or Master‘s thesis and in some cases it can be part of a practical semester lasting at least 6 months.

We currently offer a lab project for a

Master/Diploma Thesis in Molecular Cell Biology

which is suitable for highly motivated students who have strong interest in state-of-the-art skin cell culture techniques. The proposed project deals with the modulation of a newly identified ion channel family and determining its importance for skin homeostasis.

We offer the opportunity to extend your knowledge in the following methodologies:

  • Cultivation of human primary cells and cell lines (human keratinocytes)
  • Transfection and viral transduction (S2 biosafety level)
  • In vitro differentiation in 2D and in reconstituted 3D epidermis models
  • Gene expression analysis (qRT-PCR, Western blot)
  • Cell-based assays to measure ion channel activities in a high-throughput format
  • Quantitative determination of ion channel-small molecule relationships
  • Genome editing using CRISPR-Cas9 technology

We expect the following qualifications and skills:

  • M.Sc. student in (Molecular or Cellular) Biology or related studies in Life Sciences
  • Highly motivated and keen to independently push a project
  • Advanced practical experience in tissue culture handling is a must
  • Hands-on experience in one or more of the following techniques is a must: RNA preparation, qRT-PCR or Western blot
  • Theoretical background on the CRISPR/Cas9 system and/or skin physiology is advantageous

Preferred starting date would be spring/summer, ideally in April/May 2019.